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5× reaction buffer m289a  (Promega)

 
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    Promega 5× reaction buffer m289a
    5× Reaction Buffer M289a, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5%C3%97+reaction+buffer+m289a/5%C3%97+reaction+buffer+promega+m289a/pm40251891-71-13-16
    Average 90 stars, based on 1 article reviews
    5× reaction buffer m289a - by Bioz Stars, 2026-09
    90/100 stars

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    Polymerase Chain Reaction:

    Article Title: Matrigel inhibits elongation and drives endoderm differentiation in aggregates of mouse embryonic stem cells.
    Article Snippet: A minimum of 1 μg of RNA was combined with 1 μL of Oligo (dT)15 primer (C110B; Promega) and nuclease-free water to make up a total of 5 μL and incubated at 70 °C and 4 °C for 5min each. .. PCR master mix made up of 6.1 μL nuclease-free water, 4 μL 5× reaction buffer (M289A; Promega), 2.4 μL MgCl2 (A351H; Promega), 1 dNTP mix (C114B; Promega), 0.5 μL Recombinant RNasin® Ribonuclease Inhibitor (N251A; Promega), and 1 μL Reverse Transcriptase (M314A; Promega), was added to each sample. .. Thermal cycling was as follows: 25 °C for 5min, 42 °C for 60min and 70 °C for 15min. qPCR The StepOnePlusTM Real-Time PCR System was used for quantitative PCR with SYBR green PCR Master-Mix (4368708; Thermo-Fisher Scientific).

    Recombinant:

    Article Title: Matrigel inhibits elongation and drives endoderm differentiation in aggregates of mouse embryonic stem cells.
    Article Snippet: A minimum of 1 μg of RNA was combined with 1 μL of Oligo (dT)15 primer (C110B; Promega) and nuclease-free water to make up a total of 5 μL and incubated at 70 °C and 4 °C for 5min each. .. PCR master mix made up of 6.1 μL nuclease-free water, 4 μL 5× reaction buffer (M289A; Promega), 2.4 μL MgCl2 (A351H; Promega), 1 dNTP mix (C114B; Promega), 0.5 μL Recombinant RNasin® Ribonuclease Inhibitor (N251A; Promega), and 1 μL Reverse Transcriptase (M314A; Promega), was added to each sample. .. Thermal cycling was as follows: 25 °C for 5min, 42 °C for 60min and 70 °C for 15min. qPCR The StepOnePlusTM Real-Time PCR System was used for quantitative PCR with SYBR green PCR Master-Mix (4368708; Thermo-Fisher Scientific).

    Reverse Transcription:

    Article Title: Matrigel inhibits elongation and drives endoderm differentiation in aggregates of mouse embryonic stem cells.
    Article Snippet: A minimum of 1 μg of RNA was combined with 1 μL of Oligo (dT)15 primer (C110B; Promega) and nuclease-free water to make up a total of 5 μL and incubated at 70 °C and 4 °C for 5min each. .. PCR master mix made up of 6.1 μL nuclease-free water, 4 μL 5× reaction buffer (M289A; Promega), 2.4 μL MgCl2 (A351H; Promega), 1 dNTP mix (C114B; Promega), 0.5 μL Recombinant RNasin® Ribonuclease Inhibitor (N251A; Promega), and 1 μL Reverse Transcriptase (M314A; Promega), was added to each sample. .. Thermal cycling was as follows: 25 °C for 5min, 42 °C for 60min and 70 °C for 15min. qPCR The StepOnePlusTM Real-Time PCR System was used for quantitative PCR with SYBR green PCR Master-Mix (4368708; Thermo-Fisher Scientific).



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    5× reaction buffer m289a  (Promega)
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    Promega 5× reaction buffer m289a
    5× Reaction Buffer M289a, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5%C3%97+reaction+buffer+m289a/5%C3%97+reaction+buffer+promega+m289a/pm40251891-71-13-16
    Average 90 stars, based on 1 article reviews
    5× reaction buffer m289a - by Bioz Stars, 2026-09
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    5× reaction buffer promega m289a  (Promega)
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    Promega 5× reaction buffer promega m289a
    5× Reaction Buffer Promega M289a, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5%C3%97+reaction+buffer+m289a/5%C3%97+reaction+buffer+promega+m289a/pmc10508691-185-15-17
    Average 90 stars, based on 1 article reviews
    5× reaction buffer promega m289a - by Bioz Stars, 2026-09
    90/100 stars
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